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ASCP ASCP-MLT Exam Syllabus Topics:

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Listeria are non-capsulated, non-acid-fast, ß-hemolytic, gram-positive bacilli that have a characteristic tumbling motility. They are found primarily in the environment and in the human GI tract. Since L.
monocytogenes multiplies intracellularly, control of listeriosis requires cell-mediated immunity; thus, immunocompromised patients (such as newborns) are at high risk.
Which bacterial species is a common agent of neonatal bacteremia?

A) Staphylococcus aureus
B) Listeria monocytogenes
C) Enterococcus faecium
D) Streptococcus pyogenes

2. Calculation:
Cells Counted (in this case the average of both sides) X dilution factor (in this case 100) / # of squares counted (in this case 9) X area of each square (1mm2) X 0.1mm (depth factor) So, in this problem:
158 x 100 / 9 x 1 x 0.1mm = 17555.55/mm3 (can be converted to 17.5 x 109/L*)
*There are 1,000,000 mm3 in a liter (L). So 17555.55 X 1,000,000 = 17.5 x 109/L A manual white blood cell count was performed by the hematology technologist. The cell counts for both sides were 152 and 164 respectively. All nine large squares were counted on each side. The dilution for this kit was pre-measured at 1:100. What should the technologist report as the white cell count?

A) 175 x 10^9/L
B) 1.75 x 10^9/L
C) 17.5 x 10^9/L
D) 177.5 x 10^9/L

3. Antibodies are differentiated based on their heavy chains; different heavy chains create different isotypes.
Humans have five different isotypes of immunoglobulins.
Immunology
The part of the molecule responsible for differences among immunoglobulin classes is:

A) Fc
B) Hinge regions
C) light chains
D) heavy chains
E) Fab

4. Which EBV markers would be MOST likely positive for an individual who had infectious mononucleosis 9 years ago?

A) None of the markers would be positive after 9 years
B) Anti-VCA (IgG) and anti-EBNA
C) All would still be positive
D) Anti-VCA (IgM) and heterophile

5. Malbranchia species share the production of alternate staining arthroconidia as a common feature with the mold form of Coccidioides immitis.
Both Geotrichum species and Trichosporon species produce rectangular-shaped arthroconidia; however, they are regularly rather than alternately staining. Additionally, the arthroconidia of Geotrichum may produce germ tubes from one corner and the arthroconidia of Trichosporon species may produce blastoconidia from adjacent corners, features not shared by either Malbranchia species or Coccidioides immitis.
The hyphae of Microsporum canis, as seen in direct KOH mounts of skin scales, may break up into arthroconidia; however, they are much narrower in dimension and do not share the alternate staining characteristics.
Microbiology
The hyaline saprobic fungus that has microscopic features similar to the mold form of Coccidioides immitis is:

A) Malbranchia species
B) Trichosporon species
C) Geotrichum species
D) Microsporum canis

Solutions: